In the latest issue of BioTechniques, we explore a range of topics in various fields. We dive into quality control mechanisms in mitochondrial transplantation, methods for extracting RNA from formaldehyde-fixed neuromusculoskeletal tissue, and protocols for utilizing a HeLa cell extract-derived cell-free protein synthesis system to study protein complexes and RNA viruses.
The Technology News section focuses on emerging technologies for recording the brain in vivo, with a particular emphasis on exploring mental health conditions. Meanwhile, the Editorial section discusses considerations for developing mitochondrial transplantation techniques for individualized medicine.
The Reports section covers several topics, including an industry perspective approach and control strategy for the implementation of ready-to-use cells in bioassays, a multiparameter screen that optimizes immunoprecipitation, and extracting high-quality RNA from formaldehyde-fixed naturally aged neuromusculoskeletal tissues. Additionally, we present a protocol for high-throughput screening for a SARS-CoV-2 frameshifting inhibitor using a cell-free protein synthesis system.
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